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The mycelia of Tricholoma matsutake DGUM 26001, 26101, 26210 and FRI 91024 were used to determine the extracellular enzyme activity in mycelia. When the filtrate of culture broth after 30-day cultivation at 24℃ was used as a crude solution of extracellular enzyme, the average specific activity of α-amylase was 6142.3 unit/㎎?protein. The specific activity of xylanase was comparatively high. However, little or no enzyme activities were found for β-glucosidase, ligninase, CMCase, chitinase, protease, and lipase.
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